Journal: Bioactive Materials

Article Title: Collagen/hyaluronan based hydrogels releasing sulfated hyaluronan improve dermal wound healing in diabetic mice via reducing inflammatory macrophage activity

doi: 10.1016/j.bioactmat.2021.04.026

Figure Lengend Snippet: Primer sequences and annealing temperatures (T anneal ) used for qPCR.

Article Snippet: Primary antibodies used and dilutions were: anti-mouse CD11b-PE (Miltenyi, clone M1/70.15.11.5) 1:50, rat anti-mouse F4/80 (AbD Serotec, clone A3-1) 1:200, rat anti-mouse CD31 (Dianova, clone SZ31) 1:100, anti-mouse pan-cytokeratin (panCK)-FITC (Sigma, clones C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2) 1:100, anti-mouse αSMA-Cy3 (Sigma, clone 1A4) 1:200, rabbit anti-mouse Ki67 (Bethyl Laboratories, polyclonal) 1:400, rabbit anti-mouse RELMa (Peprotech, polyclonal) 1:50.

Techniques: Sequencing

Journal: Bioactive Materials

Article Title: Collagen/hyaluronan based hydrogels releasing sulfated hyaluronan improve dermal wound healing in diabetic mice via reducing inflammatory macrophage activity

doi: 10.1016/j.bioactmat.2021.04.026

Figure Lengend Snippet: Hydrogels releasing sHA accelerate re-epithelialization, tissue formation and angiogenesis in wounds in diabetic db/db mice. Wound study was performed as described in and illustrated in . A) Macroscopic appearance of wounds. B) Quantification of wound closure. C) H&E staining of wound sections. D ) IF staining of aSMA and panCK in wound sections visualizing granulation tissue and neoepithelium, respectively. E) IF staining of Ki67 in wound sections visualizing proliferating cells and quantification of Ki67+ cells. Epidermal layer and granulation tissue were defined as region of interest (ROI). F) IF staining of CD31 in wound sections visualizing newly formed vessels and quantification of connected CD31 + area. Granulation tissue was defined as ROI. G) Gene expression relative to reference gene GAPDH of angiogenic markers (VEGF/CD31) and pro-regenerative tissue markers (EGF/Ki67) in wound tissue. g = granulation tissue; e = epidermis; i = immune cell infiltrate. Arrow head marks epithelial tip. Dotted line marks border between epidermis and dermis. Scale bars: 300 μm. Each symbol represents one wound. Unpaired t -test: *p ≤ 0.05. AU = arbitrary unit. IntDen = integrated density.

Article Snippet: Primary antibodies used and dilutions were: anti-mouse CD11b-PE (Miltenyi, clone M1/70.15.11.5) 1:50, rat anti-mouse F4/80 (AbD Serotec, clone A3-1) 1:200, rat anti-mouse CD31 (Dianova, clone SZ31) 1:100, anti-mouse pan-cytokeratin (panCK)-FITC (Sigma, clones C-11+PCK-26+CY-90+KS-1A3+M20+A53-B/A2) 1:100, anti-mouse αSMA-Cy3 (Sigma, clone 1A4) 1:200, rabbit anti-mouse Ki67 (Bethyl Laboratories, polyclonal) 1:400, rabbit anti-mouse RELMa (Peprotech, polyclonal) 1:50.

Techniques: Staining, Expressing